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Very first PCR was done by using exon 4a-specific forward primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was carried out through the use of the 1st PCR product or service being a template with the nested Ex4a(+)WT1 cDNA-specific forward primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') and also the nested adaptor interior primer (